Inferring direct DNA binding from ChIP-seq
نویسندگان
چکیده
منابع مشابه
Inferring direct DNA binding from ChIP-seq
Genome-wide binding data from transcription factor ChIP-seq experiments is the best source of information for inferring the relative DNA-binding affinity of these proteins in vivo. However, standard motif enrichment analysis and motif discovery approaches sometimes fail to correctly identify the binding motif for the ChIP-ed factor. To overcome this problem, we propose 'central motif enrichment...
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Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) allows researchers to determine the genome-wide binding locations of individual transcription factors (TFs) at high resolution. This information can be interrogated to study various aspects of TF behaviour, including the mechanisms that control TF binding. Physical interaction between TFs comprises one important asp...
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Chromatin immunoprecipitation (ChIP), followed by high-throughput DNA sequencing (ChIP-seq), enables genome-wide mapping of transcription-factor binding sites (TFBS). Several transcription factors (TFs) have been known to be able to differentiate tumor sub-types in diseases like cancer. For instance, the Luminal A and Luminal B sub-types of breast cancer tumors are high in estrogen receptor (ER...
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Chromatin immunoprecipitation followed by next-generation DNA sequencing (ChIP-seq) is a widely used technique for identifying transcription factor (TF) binding events throughout an entire genome. However, ChIP-seq is limited by the availability of suitable ChIP-seq grade antibodies, and the vast majority of commercially available antibodies fail to generate usable data sets. To ameliorate thes...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2012
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gks433